How does the veterinary AccordQuick System fully automatic thin film microfluidic nucleic acid detection all-in-one machine realize one-stop nucleic acid extraction?
Publish Time: 2025-01-20
The AccordQuick System fully automatic thin film microfluidic nucleic acid detection all-in-one machine realizes one-stop nucleic acid extraction, detection and analysis through highly integrated technology and optimized process design.
1. Integrated design
Microfluidic thin film system: The device integrates thin film microfluidic technology, integrating reagent storage, sample processing, nucleic acid extraction, amplification and detection functions into one system. This design reduces manual operation steps and improves efficiency.
Modular structure: The device consists of multiple functional modules, including sample loading module, nucleic acid extraction module, amplification detection module and result analysis module, each module works together to achieve one-stop service.
2. Sample loading and pretreatment
Automated sample loading: The user only needs to add the sample (such as blood, saliva or tissue fluid) to the sample slot, and the device will automatically transfer the sample to the microfluidic system.
Sample lysis: The chemical reagents (such as cell lysis solution) in the microfluidic channel are used to quickly lyse the cells and release nucleic acids. The lysis process is achieved through precise pressure control or magnetic bead technology.
Droplet control: The microfluidic system generates tiny droplets to ensure that the nucleic acid extraction process of each sample is carried out independently to avoid cross contamination.
3. Nucleic acid extraction
Magnetic bead adsorption method: The device uses magnetic bead technology to adsorb nucleic acids. When the lysed liquid passes through the microfluidic channel, the magnetic beads specifically bind to the nucleic acid, and the remaining impurities are filtered or excluded.
Washing and purification: The reaction liquid bound to the magnetic beads is guided to the washing channel, and the impurities are removed by the washing reagent, and then the purified nucleic acid is released through subsequent steps.
Magnetic control: The built-in magnetic module is used to control the movement of the magnetic beads to ensure that the adsorption, washing and release processes of nucleic acids are completed efficiently.
4. Nucleic acid amplification and detection
Real-time fluorescence PCR amplification: The extracted nucleic acid directly enters the amplification module, and the device automatically adds PCR reaction reagents (such as primers, enzymes and fluorescent probes) and amplifies the target nucleic acid fragments through real-time fluorescence PCR.
Microfluidic design: The precise fluid control mechanism in the microfluidic channel ensures the uniform distribution of the PCR reaction liquid, avoids the generation of bubbles, and improves the amplification efficiency.
Real-time monitoring: The built-in fluorescence detection module of the device monitors the changes of fluorescence signals during the PCR reaction in real time and generates an amplification curve.
5. Result analysis and reporting
Automated data analysis: The device analyzes the fluorescence signal curve through the built-in software, automatically calculates the Ct value (cycle threshold), and judges the result (positive or negative) according to the preset threshold.
Result output: After the analysis is completed, the device automatically generates a test report and outputs the results through the display screen or the connected computer.
Data storage: The test data can be automatically stored in the device for subsequent query and remote transmission.
6. Key technologies and advantages
Highly integrated: The device integrates sample processing, nucleic acid extraction, amplification and detection into one platform without manual intervention, reducing operational errors.
Microfluidic technology: Through the precise control of the microfluidic film system, efficient management of liquids is achieved, reducing reagent consumption and contamination risks.
Fast and efficient: The entire detection process is usually completed within 30-60 minutes, which is suitable for rapid diagnosis needs.
Strong sample compatibility: Supports multiple sample types (such as blood, saliva, sputum, etc.) to adapt to different application scenarios.
Anti-pollution design: The equipment effectively prevents cross-contamination between samples through independent microfluidic channels and droplet control technology.
The AccordQuick System fully automatic thin-film microfluidic nucleic acid detection all-in-one machine achieves one-stop nucleic acid extraction, amplification and detection through highly integrated design, microfluidic technology, automated processes and real-time monitoring and other innovative technologies. Its efficient, fast and anti-pollution design makes it an important tool in clinical diagnosis, public health and food safety.
