How does the molecular diagnosis platform ensure the accuracy of the test results through the design of a fully enclosed thin film microfluidic bag?
Publish Time: 2025-01-06
The molecular diagnosis platform can effectively prevent cross-contamination between samples and ensure the accuracy of test results through the design of a fully enclosed thin film microfluidic bag.
1. Fully enclosed design
Isolated samples: The design of the fully enclosed thin film microfluidic bag completely isolates each sample in an independent bag. Each bag is a complete microfluidic system that includes steps such as sample collection, processing, amplification, and detection. This design ensures that the sample does not come into direct contact with other samples, thereby preventing cross-contamination.
Prevent external contamination: The fully enclosed design can also prevent contaminants in the external environment from entering the bag, further improving the accuracy of the test.
2. Film material
Chemical inertness: The film material usually selects chemically inert materials such as polypropylene (PP) or polyethylene (PE), which will not react with the chemicals in the sample, ensuring the integrity of the sample and the accuracy of the test results.
Biocompatibility: The film material also has good biocompatibility and will not have adverse effects on the biological molecules in the sample, ensuring the safety and reliability of the detection process.
3. Microfluidic control
Precise control: The flow of liquid in the microfluidic bag is precisely controlled by microfluidic control technology. Through the design of micropumps, valves and channels, the flow path and speed of the sample in the bag can be precisely controlled to ensure that the sample does not leak or mix.
Reduce dead volume: The microfluidic design reduces the dead volume in the bag (i.e. the volume of liquid that cannot be effectively utilized), ensuring full utilization of samples and reagents and reducing the risk of cross-contamination.
4. Disposable use
Disposable bags: Each thin film microfluidic bag is disposable and directly discarded after use. This design avoids the risk of contamination caused by reuse and ensures the independence and accuracy of each test.
No cross-use: Since each bag is independent, there will be no cross-use between different samples, further preventing cross-contamination.
5. Automated operation
Automated loading: The molecular diagnosis platform is usually equipped with an automated loading system that can accurately inject samples and reagents into each microfluidic bag without human intervention. Automated operation reduces the possibility of human error and contamination.
Automated testing: The automated testing system can monitor and record the testing process in each bag in real time to ensure the accuracy and repeatability of the test results.
6. Real-time monitoring
Real-time monitoring system: Some advanced molecular diagnosis platforms are also equipped with real-time monitoring systems that can monitor the reaction process and sample status in the bag in real time. If an abnormality is found, the system can promptly alarm and take measures to ensure the accuracy of the test results.
Through the design of a fully enclosed film microfluidic bag, the molecular diagnosis platform can effectively isolate samples, prevent cross-contamination between samples, and ensure the accuracy of the test results. This design combines the chemical inertness and biocompatibility of film materials, the precision of microfluidic control, the safety of disposable use, and the efficiency of automated operation, providing a reliable solution for molecular diagnosis.
