How does the AccordQuick system achieve a rapid testing process of sample in and result out?
Publish Time: 2025-03-04
The AccordQuick system integrates thin-film microfluidic technology and fluorescent quantitative PCR technology to achieve a rapid testing process of sample in and result out.
1. Sample processing and nucleic acid extraction
Sample injection: The user adds the sample to be tested (such as blood, throat swab, tissue, etc.) to the injection area of the AccordQuick system.
Nucleic acid extraction: The all-in-one machine uses a preset automation program inside, using the microchannel and microvalve structure on the thin-film microfluidic chip to guide the sample into the nucleic acid extraction area. In this area, the sample undergoes lysis, washing, purification and other steps to automatically complete the extraction of nucleic acid.
2. PCR amplification
Reagent mixing: The extracted nucleic acid and the reagents required for PCR amplification (such as primers, probes, enzymes, etc.) are automatically mixed on the thin-film microfluidic chip.
Amplification reaction: The mixed liquid is guided to the PCR amplification area, which realizes PCR amplification steps such as DNA denaturation, annealing and extension through precise temperature control. Fluorescence quantitative PCR technology monitors the fluorescence signal of the amplified product in real time during this process, thereby accurately reflecting the content of the target nucleic acid.
3. Result analysis
Signal acquisition: After the amplification is completed, the all-in-one machine automatically collects the fluorescence signal data.
Data analysis: Using the built-in software algorithm, the collected fluorescence signal data is processed and analyzed to obtain the test results.
Result output: After the analysis is completed, the all-in-one machine displays the test results on the screen in digital or graphical form, and can print or export the test report.
4. Technical characteristics and advantages
One-stop completion: From sample injection to result output, the entire process is automatically completed by the all-in-one machine without manual intervention.
Easy operation: Users only need simple operations to complete the test, which greatly simplifies the cumbersome steps of traditional nucleic acid testing.
Accurate detection: Using thin film microfluidics technology and fluorescence quantitative PCR technology, it can accurately detect a variety of pathogens and gene targets.
High sensitivity and strong specificity: By optimizing PCR amplification conditions and fluorescence signal detection strategies, the sensitivity and specificity of the detection are improved.
Wide applicability: It can be applied to clinical testing, environmental monitoring, food safety testing and other fields.
In summary, the AccordQuick system integrates advanced thin-film microfluidics technology and fluorescent quantitative PCR technology to achieve a rapid detection process of sample input and result output. This process not only simplifies the steps of traditional nucleic acid testing, but also improves the accuracy and efficiency of testing.
